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KMID : 0386219970090010037
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Journal of Korean Academy of Craniomandibular Disorders
1997 Volume.9 No. 1 p.37 ~ p.48
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Alterations of proteoglycan and link protein in condylar articular cartilage following surgical induction of anterior disc displacement
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최원석/Choi, Won-Seok
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Abstract
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The purpose of this study was to test the hypothesis that surgical induction of anterior disc displacement(ADD) in the rabbit craniomandibular joints(CMJ) will lead to degenerative osteoarthritic changes in the articular cartilage of the rabbit mandibular condyle by analyzing alterations of proteoglycan molecules and link protein. The quantities of cartilage proteoglycans such as chondrooitin-4-sulfate(C4S), chondroitin-6-sulfate(C6S), keratan sulfate(KS) and link protein(LP) were measured using immunogold labeling technique at the electron microscopic level.
The right joint of each of five rabbits was exposed surgically, and all discal attachments were severed except for the posterior attachment. The disc was then displaced anteriorly and sutured to the zygomatic arch. The left joint served as a sham-operated control. Ten additional joints were used as non-operated controls. Deeply anesthetized rabbits were perfused with 2% buffered formalin two weeks after surgery.
The mandibular condyles were excised and decalcified in ethylenediaminetetraacetic acid(EDTA).
Water-soluble plastic embedded sections were incubated in monoclonal antibuodies directed against C4S, C6S, KS and LP. After incubation in the appropriated colloidal gold conjugated secondary antibodies, tissue sections were studied with electron microscope.
The results of the immunocytochemistry using colloidal gold conjugates at the electron microscopic level showed statistically significant declines in C4S, C6S, KS, LP (p<0.05) in the osteoarthritic cartilage.
It is concluded that surgical induction of ADD in the rabbit CMJ leads to alterations of the proteoglycan molecules in the articular cartilage of the mandibular condyle similar to those described previously in human ADD and in osteoarthritis of other synovial joints. The results of this study provide evidence that the loss of the shock absorber function of the disc, and the exposure of the condyles to overloading may cause the injured chondrocytes to secrete degenerative cytokines as indicated by the loss of proteoglycans and LP. These molecular changes are expressed at the subcellular and cellular levels as osteoarthritis or degenerative joint disease.
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